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KMID : 0381920090390030261
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Korean Journal of Microscopy
2009 Volume.39 No. 3 p.261 ~ p.266
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Degenerative Changes of the Rat Dorsal Root Ganglion (DRG) Cells Following a Tight Spinal Nerve Ligation
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Kim Yi-Suk
Jo Seung-Mook
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Abstract
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This study aim to disclose a possible mechanism for the neuronal cell death induced by peripheral nerve injury following a spinal nerve ligation (SNL) as a neuropathic pain model. Male Sprague-Dawley rats (270~290 g) were used for this study. Pain threshold was evaluated for their response to mechanical (von Frey hairs) stimuli 1, 3, and 7 days after a tight ligation of L5 ventral ramus. In control group, the small ganglion cells were strongly stained with routine toluidine blue (TB), whereas the large ganglion cells showed a little bit weak stainity. Each large ganglion cell is surrounded by perineuronal satellite cells. In experimental groups, small ganglion cells showing apparent degenerative changes increased on 1 day, and showed a peak in degenerative cell number at 3 days group, and decreased gradually at 7 days group. We also found a small number of large-sized ganglion cells showing mild degenerative changes. However their satellite cells ware relatively intact with no typical findings throughout this experiment. Under the electron microscope, small ganglion cells showed various stage and typical features of the dark degeneration including mitochondrial swelling.
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KEYWORD
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Dark degeneration, Ganglion cell, Nerve ligation, Rat
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